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deconvolution (jdcv, 10 iterations)  (Carl Zeiss)


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    Carl Zeiss deconvolution (jdcv, 10 iterations)
    ( A and B ) Slices through tomograms collected on cryo-FIB–milled H. borinquense cells grown at 45°C and 2.45 M NaCl. Tomograms reveal extended (A) and contracted (B) T6SS-like CIS structures inside the cells. Additional examples of both extended and contracted T6SSs are also shown. Shown are 13.6-nm-thick slices through Tegunov <t>deconvolution-filtered</t> tomograms. CM, cytoplasmic membrane; SL, S-layer. Scale bars, 100 nm. ( C ) Segmentation of the cryo-tomogram depicted in (A), with the cytoplasmic membrane (CM) in red, S-layer (SL) in blue, putative storage granules in gray, and the T6SS baseplate and sheath-tube module in orange and green, respectively. ( D and E ) Subtomogram average of C6-symmetrized extended T6SS baseplate and membrane anchor using 57 particles that were extracted from filtered tomograms. Shown are 1.36-nm-thick longitudinal (D) and perpendicular slices (E). The perpendicular slice reveals contacts with the membrane, i.e., densities of six feet-like structures that are arranged around the central cage. CM, cytoplasmic membrane; SL, S-layer. Scale bar, 10 nm. ( F ) Shown is a longitudinal slice through a Gaussian-filtered subtomogram average isosurface (gray) from (D) and (E). The extended baseplate of the tCIS from Anabaena sp. PCC 7120 [PDB: 7B5H ] was fitted into the average. tCIS proteins Cis19 and Cis6 were removed because the H. borinquense CIS gene cluster lacks homologs of these proteins. Note the good fit of Cis11, which makes contact with the membrane. Cis12 and Cis13 (highlighted with asterisks) at the periphery of the baseplate may also be involved in membrane anchoring. Scale bar, 10 nm.
    Deconvolution (Jdcv, 10 Iterations), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+%28jdcv%2C+10+iterations%29/pmc11566945-379-19-19?v=Carl+Zeiss
    Average 90 stars, based on 1 article reviews
    deconvolution (jdcv, 10 iterations) - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Archaeal type six secretion system mediates contact-dependent antagonism"

    Article Title: Archaeal type six secretion system mediates contact-dependent antagonism

    Journal: Science Advances

    doi: 10.1126/sciadv.adp7088

    ( A and B ) Slices through tomograms collected on cryo-FIB–milled H. borinquense cells grown at 45°C and 2.45 M NaCl. Tomograms reveal extended (A) and contracted (B) T6SS-like CIS structures inside the cells. Additional examples of both extended and contracted T6SSs are also shown. Shown are 13.6-nm-thick slices through Tegunov deconvolution-filtered tomograms. CM, cytoplasmic membrane; SL, S-layer. Scale bars, 100 nm. ( C ) Segmentation of the cryo-tomogram depicted in (A), with the cytoplasmic membrane (CM) in red, S-layer (SL) in blue, putative storage granules in gray, and the T6SS baseplate and sheath-tube module in orange and green, respectively. ( D and E ) Subtomogram average of C6-symmetrized extended T6SS baseplate and membrane anchor using 57 particles that were extracted from filtered tomograms. Shown are 1.36-nm-thick longitudinal (D) and perpendicular slices (E). The perpendicular slice reveals contacts with the membrane, i.e., densities of six feet-like structures that are arranged around the central cage. CM, cytoplasmic membrane; SL, S-layer. Scale bar, 10 nm. ( F ) Shown is a longitudinal slice through a Gaussian-filtered subtomogram average isosurface (gray) from (D) and (E). The extended baseplate of the tCIS from Anabaena sp. PCC 7120 [PDB: 7B5H ] was fitted into the average. tCIS proteins Cis19 and Cis6 were removed because the H. borinquense CIS gene cluster lacks homologs of these proteins. Note the good fit of Cis11, which makes contact with the membrane. Cis12 and Cis13 (highlighted with asterisks) at the periphery of the baseplate may also be involved in membrane anchoring. Scale bar, 10 nm.
    Figure Legend Snippet: ( A and B ) Slices through tomograms collected on cryo-FIB–milled H. borinquense cells grown at 45°C and 2.45 M NaCl. Tomograms reveal extended (A) and contracted (B) T6SS-like CIS structures inside the cells. Additional examples of both extended and contracted T6SSs are also shown. Shown are 13.6-nm-thick slices through Tegunov deconvolution-filtered tomograms. CM, cytoplasmic membrane; SL, S-layer. Scale bars, 100 nm. ( C ) Segmentation of the cryo-tomogram depicted in (A), with the cytoplasmic membrane (CM) in red, S-layer (SL) in blue, putative storage granules in gray, and the T6SS baseplate and sheath-tube module in orange and green, respectively. ( D and E ) Subtomogram average of C6-symmetrized extended T6SS baseplate and membrane anchor using 57 particles that were extracted from filtered tomograms. Shown are 1.36-nm-thick longitudinal (D) and perpendicular slices (E). The perpendicular slice reveals contacts with the membrane, i.e., densities of six feet-like structures that are arranged around the central cage. CM, cytoplasmic membrane; SL, S-layer. Scale bar, 10 nm. ( F ) Shown is a longitudinal slice through a Gaussian-filtered subtomogram average isosurface (gray) from (D) and (E). The extended baseplate of the tCIS from Anabaena sp. PCC 7120 [PDB: 7B5H ] was fitted into the average. tCIS proteins Cis19 and Cis6 were removed because the H. borinquense CIS gene cluster lacks homologs of these proteins. Note the good fit of Cis11, which makes contact with the membrane. Cis12 and Cis13 (highlighted with asterisks) at the periphery of the baseplate may also be involved in membrane anchoring. Scale bar, 10 nm.

    Techniques Used: Membrane



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    deconvolution (jdcv, 10 iterations)  (Carl Zeiss)
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    Carl Zeiss deconvolution (jdcv, 10 iterations)
    ( A and B ) Slices through tomograms collected on cryo-FIB–milled H. borinquense cells grown at 45°C and 2.45 M NaCl. Tomograms reveal extended (A) and contracted (B) T6SS-like CIS structures inside the cells. Additional examples of both extended and contracted T6SSs are also shown. Shown are 13.6-nm-thick slices through Tegunov <t>deconvolution-filtered</t> tomograms. CM, cytoplasmic membrane; SL, S-layer. Scale bars, 100 nm. ( C ) Segmentation of the cryo-tomogram depicted in (A), with the cytoplasmic membrane (CM) in red, S-layer (SL) in blue, putative storage granules in gray, and the T6SS baseplate and sheath-tube module in orange and green, respectively. ( D and E ) Subtomogram average of C6-symmetrized extended T6SS baseplate and membrane anchor using 57 particles that were extracted from filtered tomograms. Shown are 1.36-nm-thick longitudinal (D) and perpendicular slices (E). The perpendicular slice reveals contacts with the membrane, i.e., densities of six feet-like structures that are arranged around the central cage. CM, cytoplasmic membrane; SL, S-layer. Scale bar, 10 nm. ( F ) Shown is a longitudinal slice through a Gaussian-filtered subtomogram average isosurface (gray) from (D) and (E). The extended baseplate of the tCIS from Anabaena sp. PCC 7120 [PDB: 7B5H ] was fitted into the average. tCIS proteins Cis19 and Cis6 were removed because the H. borinquense CIS gene cluster lacks homologs of these proteins. Note the good fit of Cis11, which makes contact with the membrane. Cis12 and Cis13 (highlighted with asterisks) at the periphery of the baseplate may also be involved in membrane anchoring. Scale bar, 10 nm.
    Deconvolution (Jdcv, 10 Iterations), supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+%28jdcv%2C+10+iterations%29/pmc11566945-379-19-19?v=Carl+Zeiss
    Average 90 stars, based on 1 article reviews
    deconvolution (jdcv, 10 iterations) - by Bioz Stars, 2026-07
    90/100 stars
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    Image Search Results


    ( A and B ) Slices through tomograms collected on cryo-FIB–milled H. borinquense cells grown at 45°C and 2.45 M NaCl. Tomograms reveal extended (A) and contracted (B) T6SS-like CIS structures inside the cells. Additional examples of both extended and contracted T6SSs are also shown. Shown are 13.6-nm-thick slices through Tegunov deconvolution-filtered tomograms. CM, cytoplasmic membrane; SL, S-layer. Scale bars, 100 nm. ( C ) Segmentation of the cryo-tomogram depicted in (A), with the cytoplasmic membrane (CM) in red, S-layer (SL) in blue, putative storage granules in gray, and the T6SS baseplate and sheath-tube module in orange and green, respectively. ( D and E ) Subtomogram average of C6-symmetrized extended T6SS baseplate and membrane anchor using 57 particles that were extracted from filtered tomograms. Shown are 1.36-nm-thick longitudinal (D) and perpendicular slices (E). The perpendicular slice reveals contacts with the membrane, i.e., densities of six feet-like structures that are arranged around the central cage. CM, cytoplasmic membrane; SL, S-layer. Scale bar, 10 nm. ( F ) Shown is a longitudinal slice through a Gaussian-filtered subtomogram average isosurface (gray) from (D) and (E). The extended baseplate of the tCIS from Anabaena sp. PCC 7120 [PDB: 7B5H ] was fitted into the average. tCIS proteins Cis19 and Cis6 were removed because the H. borinquense CIS gene cluster lacks homologs of these proteins. Note the good fit of Cis11, which makes contact with the membrane. Cis12 and Cis13 (highlighted with asterisks) at the periphery of the baseplate may also be involved in membrane anchoring. Scale bar, 10 nm.

    Journal: Science Advances

    Article Title: Archaeal type six secretion system mediates contact-dependent antagonism

    doi: 10.1126/sciadv.adp7088

    Figure Lengend Snippet: ( A and B ) Slices through tomograms collected on cryo-FIB–milled H. borinquense cells grown at 45°C and 2.45 M NaCl. Tomograms reveal extended (A) and contracted (B) T6SS-like CIS structures inside the cells. Additional examples of both extended and contracted T6SSs are also shown. Shown are 13.6-nm-thick slices through Tegunov deconvolution-filtered tomograms. CM, cytoplasmic membrane; SL, S-layer. Scale bars, 100 nm. ( C ) Segmentation of the cryo-tomogram depicted in (A), with the cytoplasmic membrane (CM) in red, S-layer (SL) in blue, putative storage granules in gray, and the T6SS baseplate and sheath-tube module in orange and green, respectively. ( D and E ) Subtomogram average of C6-symmetrized extended T6SS baseplate and membrane anchor using 57 particles that were extracted from filtered tomograms. Shown are 1.36-nm-thick longitudinal (D) and perpendicular slices (E). The perpendicular slice reveals contacts with the membrane, i.e., densities of six feet-like structures that are arranged around the central cage. CM, cytoplasmic membrane; SL, S-layer. Scale bar, 10 nm. ( F ) Shown is a longitudinal slice through a Gaussian-filtered subtomogram average isosurface (gray) from (D) and (E). The extended baseplate of the tCIS from Anabaena sp. PCC 7120 [PDB: 7B5H ] was fitted into the average. tCIS proteins Cis19 and Cis6 were removed because the H. borinquense CIS gene cluster lacks homologs of these proteins. Note the good fit of Cis11, which makes contact with the membrane. Cis12 and Cis13 (highlighted with asterisks) at the periphery of the baseplate may also be involved in membrane anchoring. Scale bar, 10 nm.

    Article Snippet: Collected confocal stacks were deconvolved with the Zeiss LSM Plus processing function, and the Airyscan images were processed using Zeiss deconvolution (jDCV, 10 iterations) in ZenBlue (v.3.5).

    Techniques: Membrane